![]() Stone suspensions were serially diluted in PBS from 10 -1 to 10 -6 dilutions. For this approach, pulverized stone (0.5g) was suspended in 5 mL of sterile phosphate-buffered saline (PBS) solution and mixed thoroughly on a vortex mixer for 1 min. Table 1 shows the stone samples and other pertinent information on the 11 Geodermatophilaceae isolates used in this study. The pulverized stone samples were used to isolate stone-dwelling bacteria. Crushed rock samples were reduced to a powder by grinding with a sterile mortar and pestle. Stone samples were crushed aseptically with a surface-sterilized rock hammer in a Biosafety hood. Isolation of Bacteria Associated with Stone Surfaces These samples were also used to obtain bacterial isolates for culture-dependent studies. These stone samples were used in culture-independent studies to determine the stone microbiome structure. Stone samples were obtained from historic sites in Tamil Nadu, India and at three different colonial sites in New England. This study focuses on the genomes of Blastococcus and Geodermatophilus, two genera of the family Geodermatophilaceae, of bacterial strains that were isolated from samples obtained at these sites. To supplement this metagenomic approach, a culture-dependent approach was taken to isolate Actinobacteria from two of these sites (Southern Tamil Nadu, India and New England, USA). We have been investigating the stone microbiome across a variety of lithologies three sites (North Africa, Southern Tamil Nadu, India and New England, USA) using culture-independent metagenomic approaches. Members of the family Geodermatophilaceae have also been consistently isolated from stone surfaces and interiors. Because of their hyphal nature, Actinobacteria have been considered a primary colonizer of rock that then helps promote the growth of successive microbial colonizers. Despite these seemly inhospitable conditions, stone surfaces can support microbial life and well-defined communities. Stone surfaces provide a harsh environment with limited nutrient and water availability, exposure to lethal UV irradiation, potential contact with toxic metals and metalloids, and cycles in temperature variation. Keywords: Genomes, Stones, Ruins, Climate, Geochemistry, Geodermatophilaceae, Actinobacteria. Functional analysis of the Geodermatphilaceae genomes provides insight on the stone microbiome niche. Phylogenetic analysis, digital DNA:DNA hybridization (dDDH), and comparisons of the average nucleotide identities (ANI) suggest the isolates represent novel Geodermatophilus and Blastococcus species. The genomes for the two Geodermatophilus isolates, DF1-2 and TF2-6, were 4.45 and 4.75 Mb, respectively, while the Blastococcus genomes ranged in size from 3.98 to 5.48 Mb. The genomes of the 11 Geodermatphilaceae strains were sequenced and analyzed. A culture-dependent approach was performed as a companion study with this culture-independent metagenomic analysis of these stone samples and resulted in the isolation of eleven Geodermatphilaceae strains (2 Geodermatophilus and 9 Blastococcus strains). Metagenomic analysis of stone microbiome from samples collected in New England, USA and Tamil Nadu, India identified numerous Actinobacteria including Geodermatphilaceae. ![]() Select the file that you have just downloaded and select import option Reference Manager (RIS). Draft Genomes Sequences of 11 Geodermatophilaceae Strains Isolated from Building Stones from New England and Indian Stone Ruins found at historic sites in Tamil Nadu, India. Email: louis.tisaedu Telephone: 1-60 Fax: 1-60.Įnnis NJ, Dharumadurai D, Sevigny JL, Wilmot R, Alnaimat SM, Bryce JG, Thomas WK, Tisa LS. ✉ Corresponding author: Mailing address: Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, 46 College Rd., Durham, NH 03824-2617. Present address: Department of Medical Analysis, Al-Hussein Bin Talal University, Ma'an, Jordan. Present address: Seres Therapeutics, Cambridge, MA, USA.Ħ. Department of Earth Sciences, University of New Hampshire, Durham, NH, USA.ĥ. Hubbard Center for Genome Studies, University of New Hampshire, Durham, New Hampshire, USA.Ĥ. Departments of Microbiology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.ģ. Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, New Hampshire, USA.Ģ. Ennis 1,5, Dhanasekaran Dharumadurai 1,2, Joseph L. Research Paper Draft Genomes Sequences of 11 Geodermatophilaceae Strains Isolated from Building Stones from New England and Indian Stone Ruins found at historic sites in Tamil Nadu, India
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |